[3-(Trifluoromethyl)-3H-diazirin-3-yl]coumarin as a carbene-generating photocross-linker with masked fluorogenic beacon.
نویسندگان
چکیده
A coumarin-fused diazirine photolabeling agent exhibited dramatic enhancement in fluorescence after cross-linking with the target protein. Fluorescence emission from the coumarin moiety was efficiently quenched by the diazirine group, and was then intensively recovered from photolysis treatment by irradiation with light at a wavelength of 365 nm.
منابع مشابه
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ورودعنوان ژورنال:
- Chemical communications
دوره 49 98 شماره
صفحات -
تاریخ انتشار 2013